产品展厅
产品展厅
Tightly regulated tac promoter vectors useful for the expression of unfused and fused proteins in Escherichia coli
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| Vector Name | pTrc99a | Antibiotic Resistance | Ampicillin |
| Length | 4176 bp | Type | Bacterial expression vector |
| Copy Number | High copy number | Promoter | trc |
| Cloning Method | Enzyme Cut | 5' Primer | GAGCGGATAACAATTTCACACAGG |
| 3' Primer | GATTTAATCTGTATCAGG | Expression Method | IPTG induced |
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
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