产品展厅
产品展厅
Best-in-class DNA transfection reagent
产品特点：
| Reagent |
jetOPTIMUS® DNA Transfection Reagent |
|---|---|
| Molecule delivered |
Plasmid DNA |
| Applications |
Transient and stable gene expression from plasmid DNA transfection |
| Cell types |
Adherent hard-to-transfect mammalian cell: |
| Number of transfections |
1.5 ml of jetOPTIMUS® transfection reagent is sufficient to perform 3 000 transfections in 24-well plates or 750 transfections in 6-well plates following the standard protocol |
| Storage |
Store jetOPTIMUS® at 5 °C ± 3°C. |
| Provided with |
jetOPTIMUS® Buffer |
As Transfection Experts, we know that it is essential for biologists to work with physiologically relevant cell models. For gene expression studies, transfection is the preferred technique to introduce a gene of interest in a given cell model, due to its cost-effectiveness and simplicity-of-use in comparison to physical techniques (e.g. micro-injection, electroporation).
Several types of cells (primary cells, stem cells or cancer cell lines) remain difficult to transfect with DNA for different reasons: slow-dividing rates, cell fragility and cellular defense mechanisms. To address the current limits of DNA transfection and complement our existing gene expression portfolio (jetPEI® , jetPRIME® , jetMESSENGER® ), Polyplus-transfection® engineered an innovative delivery nanoparticle: jetOPTIMUS®.
jetOPTIMUS® is a powerful transfection reagent that improves cellular uptake and endosomal escape of DNA in adherent cells (even in hard-to-transfect cells) resulting in higher transfection efficiency. In order to work in relevant physiological conditions, transfection with jetOPTIMUS® requires a minimum DNA quantity and reagent volume to keep an excellent cell viability and morphology.
