TurboFect™ 转染试剂
2022-09-20
产品详情
- 产地 美国
- 产品名称TurboFect™ 转染试剂
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- 简介
Thermo Scientific TurboFect Transfection Reagent is a highly efficient, easy-to-use, non-immunogenic transfection reagent for delivery of DNA plasmids and expression vectors into eukaryotic cells.
Features of TurboFect Transfection Reagent:
? High transfection efficiency of a wide variety of cell types.
? No optimization required for most cell lines.
? Excellent transfection efficiency in the presence or absence of serum.
? Minimal cytotoxicity.
? Ready-to-use—no need to reconstitute, dilute or manipulate.
Applications of TurboFect Transfection Reagent :
? Stable and transient transfection or co-transfection of DNA or RNA.
? Experiments with adherent or suspension cells, differentiated or undifferentiated cells.
? Gene expression studies and assays involving GFP or luciferase reporters.
TurboFect Transfection Reagent is a sterile solution of a proprietary cationic polymer. TurboFect Reagent interacts with DNA to form small, stable, highly diffusible complexes which are readily endocytosed. The reagent creates a proton-sponge effect that buffers endosomal pH by provoking massive proton accumulation and passive chloride influx. As a result, rapid osmotic swelling of the endocytosed DNA-complex causes it to rupture in the cytosol, allowing translocation of DNA to the nucleus. TurboFect Reagent is ideal for transfection of a variety of cells, including primary and difficult-to-transfect cells.
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