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病毒 RNA 纯化试剂盒
2022-09-22
产品详情
- 产地 美国
- Cas：详询
- 产品名称病毒 RNA 纯化试剂盒
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- 简介
The purified Viral RNA is suitable for use in a variety of downstream applications, including RT-PCR, qRT-PCR, and qPCR, and can be used for viral load monitoring, viral detection and genotyping. Cell-free samples are first lysed and homogenized in a strong denaturant and phenol containing buffer, which immediately inactivates RNase to ensure isolation of intact RNA. After homogenization, Buffer A is mixed with the lysate and incubated. And then the mixture is centrifuged for phase separation; the upper-layer supernatant is collected and mixed with Supplemental Reagent and ethanol. The liquid is then passed through the RNA binding Spin Column containing a silica-based membrane to which the RNA binds. Impurities are effectively removed by subsequent washing. The purified total RNA is then eluted with Elution Buffer.
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