细胞上清外泌体提取试剂盒,用于粒径和电镜检测 (大包装)
2022-09-22
产品详情
- 产地 美国
- Cas:详询
- 产品名称细胞上清外泌体提取试剂盒,用于粒径和电镜检测 (大包装)
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- 简介
Exosomes are small vesicles (30–120 nm) containing RNA and protein that are secreted by various types of cells in culture, and found in abundance in body fluids, such as blood, saliva, urine, and breast milk. Exosomes were reported to function as intercellular messengers, delivering their cargo of effector or signaling macromolecules between specific cells; however, their formation, the makeup of the cargo, and biological pathways in which they are involved remain unclear.
Exosome Isolation Kit (from cell culture media) provides a simple and reliable method of concentrating intact exosomes from cell culture media samples. By tying up water molecules, the Exosome Precipitation Reagent forces less-soluble components (i.e. exosomes) out of solution, allowing them to be collected with a brief and relatively low-speed centrifugation. Then the resuspended exosomes are purified by a Purification Column (0.22 μm).
After that, the exosomes could be used for RNA purification (for RNA sequencing, RNA Chip, RT-qPCR ), protein extraction, particle size detection and electron microscopy, etc.
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