产品展厅
酶促甲基化试剂盒
2022-09-22
产品详情
- 产地 美国
- Cas：详询
- 产品名称酶促甲基化试剂盒
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- 简介
Libraries are prepared using as little as 10 ng input DNA and the supplied NEBNext Ultra II reagents and the optimized EM-seq Adaptor. TET2 then oxidizes 5-mC and 5-hmC, providing protection from deamination by APOBEC in the next step. In contrast, unmodified cytosines are deaminated to uracils. Libraries are then amplified using a NEBNext master mix formulation of Q5U® (a modified version of Q5® High-Fidelity DNA Polymerase), and sequenced using Illumina instrumentation.
The consistently high conversion performance and minimized DNA damage with the EM-seq protocol, in combination with highly efficient Ultra II library prep, result in superior detection of CpGs with fewer sequencing reads.
Features:
·Superior sensitivity of detection of 5-mC and 5-hmC.
·Greater mapping efficiency.
·More uniform GC coverage.
·Detection of more CpGs with fewer sequence reads.
·Uniform dinucleotide distribution.
·High-efficiency library preparation, with larger library insert sizes.
·Conversion module also available separately.
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