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PCR克隆试剂盒(不带感受态细胞)
2022-09-22
产品详情
- 产地 美国
- Cas：详询
- 产品名称PCR克隆试剂盒(不带感受态细胞)
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- 简介
This PCR Cloning Kit allows simple and quick cloning of any PCR amplicon, whether the amplification reactions are performed with proofreading DNA polymerases, such as Q5® which produce blunt ends; or nonproofreading DNA polymerases, such as Taq or Taq mixes (OneTaq®, LongAmp® Taq) which produce single base overhangs. This is possible due to “invisible” end polishing components in the master mix that are active during the ligation step only if needed. The kit also allows direct cloning from amplification reactions without purification, and works well whether or not the primers used in the PCR possess 5′-phosphate groups.
·Updated to allow for in vitro transcription with both SP6 and T7 promoters.
·BsaI-site removed from ampicillin-resistant gene (allows for cloning of Golden Gate Assembly modules).
·More restriction sites added, including four 8-base cutters.
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