Cell Line Stability 

Cells will undergo genotypic changes over time resulting in reduced responsiveness in normal cell culture conditions. Genetic instability is a biological phenomenon that occurs in all stably transfected cells. Therefore, it is critical to prepare an adequate number of frozen stocks at early passages. HEK-Blue™ KO-TIFA cells should not be passaged more than 20 times to remain fully functional.

Quality Control

 ? TIFA gene knockout has been verified by DNA sequencing, RT-qPCR, and functional assays. 

? The stability for 20 passages following thawing has been verified. 

? These cells are guaranteed mycoplasma-free.

CELL LINE DESCRIPTION 

HEK-Blue™ KO-TIFA cells were generated from HEK-Blue™ Null1-v cells through the stable knockout of the TIFA gene. The parental cells derive from human embryonic kidney 293 (HEK-293) cells. HEK-Blue™ KO-TIFA cells express a secreted embryonic alkaline phosphatase (SEAP) under the control of an NF-κB-inducible promoter comprised of an IFN-β minimal promoter fused to five NF-κB and AP-1 binding sites. Levels of SEAP in the supernatant can be easily determined with HEK-Blue™ Detection, a SEAP detection cell culture medium. Unlike their parental cell line, HEK?Blue™  KO-TIFA cells do not respond to cytosolic ADP?Heptose. However, they do respond to other NF-κB inducers such as TNF-α and IL-1β. HEK?Blue™ KO-TIFA cells are resistant to Zeocin™